Tag Archives: forensic blood examination

Electrophoresis Test

By @forensicfield

What is Electrophoresis Test?

Electrophoresis is being used for the separation of various enzyme system.

There are two type of electrophoretic method have been recommended for identification of blood stains;-

  • 1] Separation and identification of hemoglobin by electrophoresis, and,
  • 2] Separation and identification of serum proteins by immunoelectrophoresis.

Immunoelectrophoresis is a two‐stage process:

  • 1.} Electrophoresis is conducted, and;
  • 2.} Immunoprecipitation using antibodies against specific proteins.

Resolution is improved by staining the proteins.

The technique is widely used to identify monoclonal immunoglobulins (M‐components) in serum and urine.

It’s Uses In Forensics

It’s a Confirmatory test for blood.

Basis Of The Test

This process involves the separation of Marker Proteins that are found on the surface of red blood cells.

Indication for the Presence of Blood

Electrophoresis  can be used to isolate and compare DNA, Blood proteins and inorganic substances.

How To Perform This Test ?

For Analyzing of Proteins

Drawbacks

The disadvantages are that gels can melt during electrophoresis, the buffer can become exhausted, and different forms of genetic material may run in unpredictable forms.

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Phenolphtalein Test

By @forensicfield

What is Phenolphthalein (Kastle – Mayer Test) ?

This test is also known as Kastle-Mayer Test.

The kastle- mayer solution is of phenolphthalein indicator solution which have been produce usually by reacting with Zn powder and ZnO.

It’s Uses In Forensics

This Test Is an Inexpensive , Easy and Realiable For Examination of Blood.

It’s Sensitivity Is 1:107 In Dilution.

It is more Sensitive than Benzidine Test.

Basis Of The Test

The basis of the test is that the peroxidase-like activity of hemoglobin in blood catalysis.

Indication for the Presence of Blood

It Produce Pink Color which Indicate Presence Of Blood.

How To Perform This Test ?

If the test is negative, it is reasonable proof that “heme” is absent.

Drawbacks

It Also Give Positive Result In The Case Of Plant Or High Oxygenated Compound.

It Also Give Positive Result for heme molecule of different species so It is hard to differentiate if blood is of human or other species.

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