The disadvantages are that gels can melt during electrophoresis, the buffer can become exhausted, and different forms of genetic material may run in unpredictable forms.
Blood is a type of biological evidence which is commonly found at the scene of crime.
Blood stains are often found at various types of crime scene, such as homicide, hit and run, assault, child abuse, rape, robbery and burglary. it also uses for DNA extraction and establishes the paternity test.
Since the discovery of the ABO system by Land-steiner in 1900, knowledge in human blood identification has expanded tremendously.
Composition
Of Human Blood
Blood is a mixture of many components:
* Cells
* Enzymes
* Proteins
* Water
* Inorganic substances (salts)
Blood contains 2 types of proteins which are-
Plasma
Corpuscles
The blood cells are of three main types:
Red Blood Cells (Erythrocytes)
White blood cells (Leucocytes)
Platelets (Thrombocytes)
Use of Blood detection in Forensic Science:
Blood is one of the most
important biological traces that are often found on the crime scene. Due to
valuable information it contains, it is considered to be a very important
forensic tool. Analysis of different aspects of bloodstains can contribute to
clarify the circumstances under which some violent crimes have been committed.
Such crucial information can point criminal investigation in the right
direction and help solve the crime. In some cases it can also help with legal
determination of criminal offense which can lead to more accurate and more
appropriate punishment for the perpetrator. It is very important to determine
the sequence of events during the commitment of a violent crime involving
blood.
Identification Of Source Of Blood
Foetal blood– presence of HbF or Alpha Protein.
Blood of Pregnancy and abortion – Chorionic Gonadotropins or or Alkaline Phosphatase.
Venous blood– dark in color, oozes gradually and no spurting.
Arterial – bright red and copious and showed spraying.
Methods For The Identification Of Blood
5 Types Of Blood Test Or Methods Occurred-
Visual / Physical examination
Microscopic examination
Chemical Examination
Spectroscopic Examination
Serological examination
Visual / Physical Examination
The search for the
presence of blood at a crime scene is normally done by close visual
examination. The possibility exists, however, that blood may be present
in amounts too little to see with the unaided eye, or that the blood at the
scene had been “cleaned up” prior to arrival of the crime scene team.
Fresh stains – bright in colour, turns to brown and black after some time. Fresh stains soluble in water or in saline
Ante-mortem blood Stains – Removed in scales due to presence of fibrin.
Post-mortem blood stain – changes in powdered on removal.
Microscopic
Examination
Fresh blood stain in Microscope – Erythrocytes and leukocytes.
Microscopic appearance of cells found in a stain extract may
reveal other information.
If blood stains in an environment with a higher solute
concentrate, water leaves the cell by osmosis and the cells shrink and change
shape.
If stains are fresh it is possible to reconstitute the stain
and proceed with microscopical identification of cellular components.
