1. Chromatography is a physical method that is used to separate__________
(a) Simple mixtures
(b) Complex mixtures
(c) Viscous mixtures
(d) Metals
Answer: b
2. Which force is involved in the Chromatography?
(a) Hydrogen bonding
(b) London force
(c) Electric static force
(d) All of the above
Answer: d
3. Ion exchange chromatography is based on the_____________
(a) Electrostatic attraction
(b) Electrical mobility of ionic species
(d) Partition chromatography
Answer: a
4. Chromatography with solid stationary phase is called_________
(a) Circle chromatography
(b) Square chromatography
(c) Solid chromatography
(d) Adsorption chromatography
Answer: d
5. A combination of paper chromatography and electrophoresis involves______________
(a) Partition chromatography
(b) Electrical mobility of the ionic species
(c) Both (a) and (b)
(d) None of these
Answer: c
6. The pattern on the paper in chromatography is called
(a) Chroming
(b) Chroma
(c) Chromatograph
(d) Chromatogram
Answer: d
7. In reverse phase chromatography, the stationary phase is made______________
(a) Non-polar
(b) Polar
(c) Both a and b
(d) None of these
Answer: a
8. The components which have a small value of K have an affinity for______________
(a) Mobile phase
(b) Stationary phase
(c) No phase
(d) Solution
Answer: b
9. Which technique is also known as colour writing ?
(a) NMR
(b) Mass spectroscopy
(c) Chromatography
(d) All of the above
Answer: c
10. Which of the following HPLC detectors is used as a bulk property or general purpose detector?
(a) Electrochemical detector
(b) Fluorescence detector
(c) UV-Visible detector
(d) Evaporative Light scattering detector
Answer: d
11. Thin layer chromatography is__________
(a) Partition chromatography
(b) Electrical mobility of ionic species
(c) Adsorption chromatography
(d) None of the above
Answer: c
12. In which chromatography stationary phase is more polar than mobile phase?
(a) Ion exchange chromatography
(b) Normal phase chromatography
(c) Reversed chromatography
(d) Size exclusion chromatography
Answer: b
13. Which of the following is used as a spraying reagent in paper chromatography?
(a) Conc. HCl
(b) NaCl solution
(c) Ninhydrin solution
(d) CuSO4 solution
Answer: c
14. In gas chromatography, the basis for separation of the components of the volatile material is the difference in________
(a) Partition coefficients
(b) Conductivity
(c) Molecular weight
(d) Molarity
Answer: a
15. In which type of chromatography, the stationary phase is held in a narrow tube and the mobile phase is forced through it under pressure?
(b) Planar chromatography
(d) Gas chromatography
Answer: a
16. In chromatography, the stationary phase can be ___________ supported on a solid.
(a) Solid or liquid
(b) Liquid or gas
(c) Solid only
(d) Liquid only
Answer: a
17. What is Eluent?
(a) It is a liquid solution
(b) It is a liquid solution that is a result from Elution.
(c) It is a solvent that used for separation of absorbed material from stationary phase.
(d) None of the above
Answer: c
18. In chromatography, which of the following can the mobile phase be made of?
(a) Solid or liquid
(b) Liquid or gas
(c) Gas only
(d) Liquid only
Answer: b
19. Chromatogram is__________
(a) Solute concentration vs Elution time
(b) Solute concentration vs Elution volume
(c) a and b
(d) None of the above
Answer: c
20. In thin layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________
(a) Solid, liquid
(b) Liquid, liquid
(c) Liquid, gas
(d) Solid, gas
Answer: a
21. What is the Analyte ?
(a) Substance for separation
(b) Substance for impurity
(c) a and b
(d) None of the above
Answer: a
22. Which of the following is used as a carrier gas in gas chromatography__________
(a) Carbon dioxide
(b) Oxygen
(c) Helium
(d) Methane
Answer: c
23. In size exclusion chromatography, solute molecules are separated based on _________
(a) Molecular geometry and size
(b) Molecular composition
(c) Molecular phase
(d) Molecular formula
Answer: a
24. Ion exchange chromatography is based on?
(a) Electrostatic attraction
(b) Electrical mobility of ionic species
(c) Partition chromatography
(d) Adsorption chromatography
Answer: b
25. In Thin layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________
(a) Solid, liquid
(b) Liquid, liquid
(c) Liquid, gas
(d) Solid, gas
Answer: a
26. The basis of the technique of chromatography for separating components of a mixture is?
(a) The differing movement of particles of different mass in an electrical field
(b) The interaction of the components with a stationary and a mobile phases
(c) The absorption of infrared radiation by the components.
(d) The deflection of charged particles in a magnetic field.
Answer: b
27. HPLC is an abbreviation for?
(a) High Profit Liquid Chromatography
(b) High Performance Liquid Chromatography
(c) Higher Performance Low Chromatography
(d) Higher Profit Low Chromatography
Answer: b
28. Which of the following techniques would be most useful to identify as well as quantify the presence of a known impurity in a drug substance?
(a) NMR
(b) MS
(c) IR
(d) HPLC
Answer: d
29. Liquid chromatography can be performed in which of the following ways?
(a) Only in columns
(b) Only on plane surfaces
(c) Either in columns or on plane surfaces
(d) Neither in columns nor on plane surfaces
Answer: c
30. The process of passing a mobile phase through a chromatography column is called which one of the following?
(a) Flushing
(b) Washing
(c) Elution
(d) Partitioning
Answer: c
31. Which of the following steps takes place after injection of feed in Column chromatography?
(a) Detection of components
(b) Separation in the column
(c) Elution from the column
(d) Collection of eluted component
Answer: b
32. What happens during the ‘elution from the column’ phase in chromatography?
(a) Components with greatest affinity elute first
(b) Components with least affinity elute first
(c) Components elute in a random manner
(d) Components elute according to their concentration in the mixture
Answer: b
33. In chromatogram, the position of peaks on the time axis can be used to determine which of the following?
(a) Components of the sample
(b) Amount of component in the sample
(c) Column efficiency
(d) Column resolution
Answer: a
34. In chromatogram, the area under the peak can be used to determine which of the following?
(a) Components of the sample
(b) Amount of component in the sample
(c) Column efficiency
(d) Column resolution
Answer: b
35. Using Chromatogram as detector in Chromatography, a graph is obtained between ____________ and time.
(a) Quantity
(b) Density
(c) Concentration
(d) Specific gravity
Answer: c
36. In older analytical methods, which of the following methods were used to allow movement of the mobile phase?
(a) Pumps
(b) Pressure
(c) Gravity
(d) Blowing air into the column
Answer: c
37. For the separation of which of the following substances, Gas-solid chromatography is being used?
(a) Thermally stable organic components
(b) Volatile organic components
(c) Thermally stable inorganic components
(d) Low molecular weight gaseous species
Answer: d
38. Which of the following is not a feature of carrier gas used in gas chromatography?
(a) It must be chemically inert
(b) It should be suitable for the detector employed
(c) It should not be completely pure
(d) It should be cheap
Answer: c
39. Which of the following is the disadvantage of hydrogen, which can be used as carrier gas in gas chromatography?
(a) Dangerous to use
(b) Expensive
(c) Reduced sensitivity
(d) High density
Answer: a
40. Which of the following is the disadvantage of helium, which can be used as carrier gas in gas chromatography?
(a) Dangerous to use
(b) Expensive
(c) Reduced sensitivity
(d) High density
Answer: b
41. Which of the following is the disadvantage of nitrogen, which can be used as carrier gas in gas chromatography?
(a) Dangerous to use
(b) Expensive
(c) Reduced sensitivity
(d) High density
Answer: c
42. In which of the following methods are liquid samples injected into the column in gas chromatography?
(a) Gas tight syringe
(b) Micro-syringe
(c) Rotary sample valve
(d) Solid injection syringes
Answer: b
43. What must be done to the solid samples for it to be introduced into the column without using solid injection syringes in gas chromatography?
(a) Introduced in hot-zone of the column
(b) Dissolved in volatile liquids
(c) Introduced using rotary sample valve
(d) Introduced using sampling loops
Answer: b
44. Which of the following is the commonly used support material for the packed column in gas chromatography?
(a) Glass
(b) Metal
(c) Diatomaceous earth
(d) Stainless steel
Answer: c
45. Which of the following is the advantage of a straight packed column?
(a) It can be packed uniformly
(b) It can be repacked easily
(c) It is compact
(d) It is easier to heat it evenly
Answer: c
46. Which of the following is the disadvantage of coiled or helical shaped packed chromatographic column?
(a) It cannot be packed uniformly
(b) It cannot be repacked easily
(c) It is not compact
(d) It is not easy to heat it evenly
Answer: b
47. Capillary columns are open tubular columns constructed from which of the following materials?
(a) Glass
(b) Metal
(c) Stainless steel
(d) Fused silica
Answer: d
48. Which of the following is not a desirable feature of the ovens used in gas chromatography?
(a) It must have a fast rate of heating
(b) Power consumption should be kept low
(c) It must have maximum thermal gradients
(d) It should have proper insulation
Answer: c
49. Which of the following is the disadvantage of reciprocating pump used in liquid chromatography?
(a) Produces pulsed flow
(b) Corrosive components
(c) Does not have small hold-up value
(d) Does not have moderate flow rate
Answer: a
50. Which of the following is not a disadvantage of Pneumatic pumps used in liquid chromatography?
(a) Pulsed output
(b) Dependent on solvent viscosity
(c) Dependent on back pressure
(d) Inconvenient for solvent gradient elution
Answer: a
52. Which of the following is not a desired characteristic of pulse dampers or flow smootheners used in liquid chromatography?
(a) Easy mobile phase change over
(b) Constant flow must be maintained
(c) Should be effective at low system pressure
(d) Maximal dead volume
Answer: d
53. Which of the following will improve the efficiency of the separation process in liquid chromatography?
(a) Increase in sample size, increase in column diameter
(b) Reduction in sample size, increase in column diameter
(c) Increase in sample size, reduction in column diameter
(d) Reduction in sample size, reduction in column diameter
Answer: d
54. Which of the following are the practical problems that arise due to the decrease in column diameter?
(a) Requirement of large particle size and high pressure drop
(b) Requirement of large particle size and low pressure drop
(c) Requirement of small particle size and high pressure drop
(d) Requirement of small particle size and low pressure drop
Answer: c
55. Which of the following is not true about the guard column used in liquid chromatography?
(a) It filters particles that clog the separation column
(b) It extends the lifetime of separation column
(c) It allows particles that cause precipitation upon contact with stationary or mobile phase
(d) The size of packing varies with the type of protection needed
Answer: c
56. Which of the following columns are not used in liquid or high performance liquid chromatography?
(a) Analytical column
(b) Separation column
(c) Guard column
(d) Capillary column
Answer: d
57. Which of the following is not a Column-type Liquid chromatography?
(a) Gel permeation
(b) Ion exchange
(c) Liquid-solid
(d) Paper
Answer: d
58. Which of the following is not true about the radial compression column when compared to standard separation column?
(a) Internal diameter decreases
(b) Overall operating pressure decreases
(c) Analysis time decreases
(d) Solvent flow increases
Answer: a
59. Which of the following is not true about narrow bore column when compared to standard columns?
(a) Internal diameter decreases
(b) Volumetric flow decreases
(c) Solvent cost is saved
(d) Detector response time increases
Answer: d
60. Which of the following types of liquid chromatography uses immobilized biochemical as a stationary phase?
(a) Ion exchange chromatography
(b) Exclusion chromatography
(c) Affinity chromatography
(d) Gel permeation chromatography
Answer: c
61. Which of the following is not true about High Performance Liquid Chromatography (HPLC)?
(a) It requires high pressure for the separation of the specious
(b) There is no need to vaporise the samples
(c) It is performed in columns
(d) It has high sensitivity
Answer: b
62. Which of the following is not an advantage of Syringe type pumps used in High Performance Liquid Chromatography?
(a) Independent of viscosity
(b) Pulse-less flow
(c) High pressure capability
(d) Unlimited solvent capacity
Answer: d
63. Which of the following is not true about solvent programming which is done in high performance liquid chromatography?
(a) It provides unequal bandwidths
(b) It provides fast overall separation
(c) It provides maximum resolution
(d) It provides maximum sensitivity
Answer: a
64. Which of the following pulse damper takes up some amount of the pulsation energy which is released to provide smooth pressure without pulsations?
(a) Flexible bellows or compressible gas passed through tee columns
(b) Flexible inert diaphragm
(c) Electronic pulse damper
(d) Electrical pulse damper
Answer: a
65. Which of the following is not a characteristic of the syringe pump used in High Performance Liquid Chromatography?
(a) Pressure capability is high
(b) Maintenance is frequent
(c) Limited reservoir capability
(d) Slight change of flow rate when extremely high pressure compresses the solvent
Answer: b
66. Syringe pumps used in High Performance Liquid Chromatography are most suitable for which of the following columns?
(a) Capillary columns
(b) Guard columns
(c) Short-fast columns
(d) Small bore columns
Answer: d
67. Which of the following cannot be done to reduce ripple in High Performance Liquid Chromatography?
(a) Using bellows
(b) Using restrictors
(c) Using long nylon tube between pump and column
(d) Avoiding the use of the solvent pump
Answer: d
68. Which of the following is not true about Hydraulic capacitance flow control system used in HPLC?
(a) It can be used only for liquids with low viscosity
(b) It is irrespective of solvent compressibility
(c) It maintains a constant flow
(d) It smoothens high pressure pump pulsations
Answer: a
69. In gas-liquid chromatography, when films are used in the interior of the capillary column, then what is the value of Eddy diffusion?
(a) Greater than 1
(b) Less than 1
(c) Zero
(d) Less than zero
Answer: c
70. What is the drawback that occurs in using ion exchange chromatography on sulphonated polystyrene resin and colourimetry for amino-acid analysis?
(a) Less accuracy
(b) Low resolution
(c) Inconvenient to handle many individual samples
(d) Slow in operation
Answer: c
71. Which one of the following methods is the most suitable for amino-acid analysis?
(a) Gas chromatography
(b) Ion exchange chromatography
(c) Paper electrophoresis
(d) Resin column chromatography
Answer: d
72. Which of the following colour reagents are used in Resin column chromatography?
(a) Marquis reagent
(b) Benedict reagent
(c) Ninhydrin
(d) Nessler’s reagent
Answer: d
73. Which of the following amino-acids is measured at a wavelength of 440nm using photometric systems?
(a) Proline
(b) Alanine
(c) Glutamine
(d) Valine
Answer: a
74. In Automatic amino-acid analyzer, the sample containing ___________ of each amino compound is introduced at the top of the ion exchange column.
(a) 1 to 10 µmoles
(b) 1 to 10 moles
(c) 0.05 to 2 moles
(d) 0.05 to 2 µmoles
Answer: d
75. Which of the following cannot be analysed using resin column chromatography?
(a) Peptides
(b) Amines
(c) Amino compounds
(d) Components which are ninhydrin negative
Answer: d
76. Trapped air in pumps of Automatic amino-acid analyzer is eliminated using which of the following ways?
(a) Suction pump
(b) De-aerator
(c) Overcome naturally by pump pressure
(d) Using pulse damper
Answer: b
77. What is the drawback that occurs in using gas chromatography for amino-acid analysis?
(a) Costly
(b) Cannot be used for proteins or polypeptides
(c) Slow process
(d) Complex in operation
Answer: b
